In recent years, increase of food demand due to global overpopulation has been a burden for agriculture, which is causing the overuse of chemical pesticides and fertilizers. Consequently, the ground water, air and soil have been polluted with industrial substances such as potassium (K), phosphorus (P) and nitrogen (N) which cause serious threat to human health and environment. Over-use of ammonium sulphate causes the decline of soil fertility due to the lowering of soil pH. In addition, overuse of inorganic nitrogen and phosphate releases the harmful compound into the soil, water and atmosphere, a typical example is phosphorus and nitrate eutrophication that cause death of animal and the dense growth of plant by lack of oxygen. In general, plants absorb only 10-40% of total fertilizer applied. In other words, 60% to 90% of fertilizer is lost. To improve this problem, microbial inoculation is necessary to manage integrated nutrient for green environment and sustainable agricultural production. In the innovative point of view, the alternatives to agro-chemicals are biological products based on organic fertilizer. Bio-fertilizer is defined as a biological product that contains living organisms to support for plant growth and improve the natural fertility of soil. There are two forms of bio-fertilizers, the solid and liquid form. The good carriers for the solid form should be low cost, nontoxic, easy to sterilize, available in sufficient amount, good absorption and maintenance of moisture, free of lump-forming and easy to process, suitable for seed germination and good at buffering capacity of pH [5-6]. Liquid bio-fertilizer is considered as the potential strategy to increase the shelf-life of bio-fertilizer. Beneficial microorganisms in bio-fertilizer can improve the nutrient in soil by their potential of nitrogen fixation, phosphorus metabolisms and provide plant hormones. Bio-fertilizer has been applied not only to enrich macro- and micro- nutrient of soil but also to support plant growth and to increase soil organic matter by releasing bio-degradation and antibiotics. Bio-fertilizer can be applied to soil or seed inoculants, for multiple crop production and nutrient cycling. The additional benefits of the use of bio-fertilizer are no side effects to ecosystem even prolonged application of organic substances.